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Abstract #0546

Validation of Cardiac Diffusion Tensor MRI using Transparent Tissue Preparation (CLARITY) with 3D Optical Microscopy

Christopher Nguyen1, Sang-Eun Lee2, Jongjin Yoon3, Hyuk-Jae Chang2, Sekeun Kim2, Chul Hoon Kim3, and Debiao Li1,4

1Biomedical Imaging Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA, United States, 2Division of Cardiology, Yonsei University College of Medicine, Seoul, Korea, Republic of, 3Departement of Pharmacology, Yonsei University College of Medicine, Seoul, Korea, Republic of, 4Bioengineering, University of California Los Angeles, Los Angeles, CA, United States

The myocardium consists of a complex 3-dimensional (3D) microstructure has been shown to be perturbed in the presence of myocardial ischemia. Recently, diffusion tensor magnetic resonance imaging (DT-CMR) was introduced which can characterize the 3D tissue microstructure in intact myocardium. However, past histologic validation of DTI has been limited since traditional pathology allows only 2D optical microscopy after potentially destructive tissue sectioning. We present a novel approach to validate the derivation of the myocardial fiber orientation (MFO) using DT-CMR with 3D histology using a non-destructive, transparent-tissue preparation technique (CLARITY). Results indicate MFO derived from 3D histology and DT-CMR are strongly concordant.

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