Synopsis: We demonstrate the capability of unique ‘cargo internalization receptors’ (CIR) for specific cell tracking. A nanobody for green fluorescent protein (GFP) was used to engineer surface receptors which undergo rapid internalization after GFP binding. For 19F MR visibility, the GFP carrier was equipped with perfluorocarbon (PFC) contrast cargo. PFC cargo uptake after CIR transfection was verified by flow cytometry, confocal microscopy, and 1H/19F MRI. The results show that this approach can be successfully used for targeted contrast agent internalization, which can be extended to an cell-specific CIR expression in transgenic mice for in vivo cell tracking by 19F MRI.
How to access this content:
For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.
After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.
After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.
Keywords