Abstract #0768

Bioreactor for in vitro optical fluorescence and magnetic resonance spectroscopy

Benjamin L Cox^1,2,3, Joseph M Szulczewski^3,4, Kai D Ludwig¹, Erin B Adamson¹, Robert A Swader², Sarah A Erickson-Bhatt^2,3,4, Patricia J Keely⁴, Kevin W Eliceiri^1,2,3,5, and Sean B Fain^1,6

¹Medical Physics, University of Wisconsin - Madison, Madison, WI, United States, ²Medical Engineering, Morgridge Institute for Research, Madison, WI, United States, ³Laboratory for Optical and Computational Instrumentation (LOCI), University of Wisconsin - Madison, Madison, WI, United States, ⁴Cell and Regenerative Biology, University of Wisconsin - Madison, Madison, WI, United States, ⁵Biomedical Engineering, University of Wisconsin - Madison, Madison, WI, United States, ⁶Radiology, University of Wisconsin - Madison, Madison, WI, United States

The design and implementation of a novel bioreactor capable of facilitating both magnetic resonance spectroscopy (MRS) and optical fluorescence microscopy for complementary metabolic information is described. Fluorescence lifetime imaging (FLIM) of nicotinamide adenine dinucleotide (NADH) and hyperpolarized [1-¹³C] pyruvic acid (PA) MRS of human breast cancer cells in a 3D collagen matrix is demonstrated. The system provides a novel test-bed for simulating cell-matrix and cell-cell interactions in a 3D microenvironment for investigating multi-scale cellular metabolism in vitro.

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