Circumferential strain (Ecc) derived from cardiac DENSE 3D displacement maps is a promising biomarker for diagnosing early stages of cardiac disease. Ecc is commonly evaluated only in the mid-wall where it is expected to align with the local myofiber direction. Our aim was to combine cDTI and DENSE displacement data to directly characterize strain along the fiber direction (Eff) throughout the heart. Across all healthy subjects (N=9), Eff had a smaller peak systolic transmural gradient than Ecc (-0.035±0.041 vs. -0.097±0.030 (unitless), p<0.001). Eff is a more spatially uniform measure of regional LV function in healthy volunteers and provides a microstructurally anchored measure of cardiomyocyte performance.