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Abstract #1437

Optimal contrast enhancement of blockface images for MRI guided reconstruction of mouse brain volumes

Christoffer Gothgen1, Catharina Maria Holland1, Christos Zoupis Schoinas1, Karine Mardon2, Maciej Plocharski1, Lasse Riis Ƙstergaard1, and Andrew Janke2

1Department of Health Science and Technology, Aalborg University, Aalborg, Denmark, 2Centre for Advanced Imaging, University of Queensland, Brisbane, Australia

Blockface imaging can improve atlases of the rodent brain by supplying high resolution images. This study compares three different contrast stretching methods for enhancing the information in blockface images together with a registration to a 16.4 T atlas of the mouse brain. Contrast enhancement technique used was histogram equalization, adjusting the image intensity values by stretching them between the bottom 1% and the top 1% and CLAHE with a clip limit of 0.01 and a uniform histogram. Registrations was rigid, affine and SyN. By using CLAHE as contrast stretching method a high similarity to the MRI was found.

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