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Abstract #1968

Functional and structural ex vivo “MRI staining” using manganese-enhanced MRI (MEMRI), Gd-DTPA and Mn-Gd mixture

Chika Sato1, Kazuhiko Sawada2, David Wright3,4, Tatsuya Higashi1, and Ichio Aoki1

1Molecular Imaging and Theranostics, National Institute of Radiological Sciences, QST, Chiba, Japan, 2Department of Nutrition, Faculty of Medical and Health Sciences, Tsukuba International University, 3Anatomy and Neuroscience, The University of Melbourne, 4The Florey Institute of Neuroscience and Mental health

To reveal brain 3D microstructures noninvasively and microscopically using MRI, we developed “ex vivo MEMRI” and Mn-Gd double-contrast methods, and then compared them with conventional ex vivo Gd-DTPA-doped contrast. Because MEMRI sample loses contrast after perfusion fixation, we examined the stability of Mn2+ accumulation in ex vivo tissue samples. In addition, we tried to improve the contrast of MEMRI in combination with Gd-DTPA. The Mn-Gd double-contrast showed novel contrast and improved visibility. The functional “MRI staining” methods we developed in this study will be useful for visualizing whole brain 3D microstructures with a higher throughput compared to histological staining.

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