Abstract #1968

Functional and structural ex vivo “MRI staining” using manganese-enhanced MRI (MEMRI), Gd-DTPA and Mn-Gd mixture

Chika Sato¹, Kazuhiko Sawada², David Wright^3,4, Tatsuya Higashi¹, and Ichio Aoki¹

¹Molecular Imaging and Theranostics, National Institute of Radiological Sciences, QST, Chiba, Japan, ²Department of Nutrition, Faculty of Medical and Health Sciences, Tsukuba International University, ³Anatomy and Neuroscience, The University of Melbourne, ⁴The Florey Institute of Neuroscience and Mental health

To reveal brain 3D microstructures noninvasively and microscopically using MRI, we developed “ex vivo MEMRI” and Mn-Gd double-contrast methods, and then compared them with conventional ex vivo Gd-DTPA-doped contrast. Because MEMRI sample loses contrast after perfusion fixation, we examined the stability of Mn²⁺ accumulation in ex vivo tissue samples. In addition, we tried to improve the contrast of MEMRI in combination with Gd-DTPA. The Mn-Gd double-contrast showed novel contrast and improved visibility. The functional “MRI staining” methods we developed in this study will be useful for visualizing whole brain 3D microstructures with a higher throughput compared to histological staining.

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