Intravoxel incoherent motion (IVIM) model provides both pure water motion and microcirculation by using multiple b values. IVIM imaging has been shown to be useful for assessment of liver diseases, including liver fibrosis and hepatocellular carcinoma. However, the clinical implementation of IVIM imaging is limited by long acquisition time. We compared IVIM imaging with different b-values to determine the combination of b-values in IVIM imaging that allows the relatively short acquisition time to obtain reproducible values of the IVIM parameters in patients with hepatocellular carcinoma. Our results showed at least 10b-values should be used in IVIM imaging for the assessment of hepatocellular carcinoma, and 5b-values IVIM imaging might increase errors in the perfusion-related f and D* values.