The aim of this study is to evaluate the feasibility of amide proton transfer-weighted (APTW) MR imaging to detect cerebral abnormalities in patients with obsessive-compulsive disorder (OCD) and to explore its clinical utility. Five OCD patients and 9 normal healthy controls (NC) underwent APTW MR imaging. The magnetic resonance ratio asymmetry (MTRasym) values at 3.5ppm of anterior cingulate cortex (ACC) and thalamus were measured on axial APTW images. We found a trend of increased MTRasym(3.5ppm) or APTW within ACC in OCD patients compared with controls. No significant difference was found between groups in the MTRasym(3.5ppm) within bilateral thalamus. Our results suggest that APTW imaging maybe a promising approach to investigate pathological changes underlying OCD and may provide insights into clinical diagnosis of OCD.