Organotypic slices are excellent controlled systems for investigating neural activity without many of the confounds introduced by in-vivo conditions. Here we describe a new setup that allows imaging of a living slice inside a MRI scanner while being stimulated with optogenetics which induces neuronal activity in pre-targeted neurons. This setup allows the survival of slices for at least three hours, without requiring new artificial CSF (aCSF) to be flushed. This property is highly advantageous for avoiding artifacts related with bubbles, motion or variation of shims during extended acquisitions.
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