This study assessed the feasibility of 2D UTE-based MR thermometry of frozen tissue during in vivo MRI-guided cryoablation. Axial 2D UTE images were acquired at the end of the first and second freeze cycle during an MRI-guided renal cryoablation procedure. Measurable MR signal could be obtained from frozen tissue. MR temperature maps were estimated using a relative signal level calibration performed in ex-vivo porcine muscle. Our work demonstrates the feasibility of 2D UTE-based MR thermometry of frozen tissue during in vivo MRI-guided cryoablation, which could be an important step towards clinical application of this technique.