The time constraints of in vivo diffusion MRI require a compromise to be made between spatial and angular resolution. Given the lack of ground truth on the configuration of human brain connections, determining the optimal operating point along this trade-off is still an open problem. We use high-SNR ex vivo human data at microscopic resolution to study the effect of spatial and angular resolution on dMRI tractography accuracy. Our findings show that voxel size has a much more dramatic effect on tractography reconstruction of challenging white matter configurations than the number of gradient directions.
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