We report here a study of MT and enhanced MT (eMT), using dual-sided RF saturation, in model membrane system to measure lipid dynamics and structure in systems of known lipid order. The eMT signal generated by dual-sided RF saturation of phospholipid vesicles is well described by Gaussian line shape and T2b is easily determined. T2b of the lipids is related to the lipid order with T2b(liquid disorder) > T2b(liquid order) > T2b(solid order). These tools provide a method to estimate lipid order in vivo.
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