This study aims to reveal the relation between traumatic microbleeds (TMBs) on susceptibility weighted imaging and diffuse axonal injury (DAI). Regional TMB and diffusion tensor imaging (DTI) data were collected from 38 traumatic brain injury patients, and 20 control subjects. Analyses included multiple linear regression among TMB parameters, clinical variables and DTI data. Only basal ganglia area TMBs were found to be significantly related to DTI alteration indicating DAI. In general, TMBs might be rather due to microvascular vulnerability than actual DAI, however, specifically basal ganglia area TMBs might be still regarded as markers of DAI.