This study was aimed to evaluate the differences between PD motor subtypes of GABA+ levels using MEGA-PRESS. PD patients were classified into PIGD and TD groups; sixteen healthy controls were recruited. All subjects underwent 3T MR examination including MEGA-PRESS. We found that GABA+ concentration was lower in PD compared with controls; furthermore, the TD group was lower than PIGD. In PD patients, the GABA+ levels were correlated with UPDRS scores. The results suggest that GABAergic dysfunction may play an important role in the pathogenesis of Parkinson’s disease. MEGA-PRESS provides a valuable examination method to discriminate between PD motor subtypes.