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Abstract #4859

Whole body, high throughput mouse embryo 3D phenotyping using multi gradient echo and ultra short echo time with computed tomography validation.

Orlando Aristizabal1, Dung Minh Hoang2, Sebastian Mendoza3, Daniel H. Turnbull2, and Youssef Zaim Wadghiri2

1Structural Biology, Skirball Institute of Biomolecular Medicine, New York, NY, United States, 2Radiology, NYU School of Medicine, 3NYU School of Medicine

In this study a combination of 3D multi gradient echo (MGE) and ultrashort echo time (UTE) 100 micron isotropic resolution where acquired from 6 fixed mouse embryos at embryonic day 16. The embryos where littermates from an engrailed knockout mouse whose mutant embryos die at birth. The data from the UTE was validated with a high resolution CT scan. Results verify the utility of this approach to image both soft tissue and the skeletal system in a high throughput manner. The expected phenotype was easily identifiable and the 3D reconstruction of the skeletal system was equivalent to CT.

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