High sampling rate is pivotal for differentiating neuronal-related from spurious correlations in resting-state-fMRI (rsfMRI). Acquiring multi-echoes (ME) during an EPI readout increases contrast-to-noise, but it can compromise temporal resolution even when combined with multiband (MB). Therefore, whether MBME-EPI is ultimately beneficial for rsfMRI remains unclear. To address this, we collected data at 3T with 2-mm resolution using MBME-EPI and the human-connectome-project MB-single-echo-EPI. Data were evaluated for spectral amplitude, consistency and specificity. MBME-EPI showed significant gains in all quantities when physiological noise and sampling rate were matched between time-series. However, there was no clear gain when different sampling rates were considered.
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