Reliable in vivo assessment of human substantia nigra (SN) requires highly trained operators and different MRI sequences. Advanced techniques have recently facilitated SN identification, but their acquisition in routine clinical practice may not be feasible. MP2RAGE allows for quantitative T1 mapping with an acceptable acquisition time (< 10 minutes). Moreover, SN can be seen on T1 maps, but not on standard MPRAGE. In this study, we tested the feasibility of semi-automated SN identification on MP2RAGE-derived T1 maps by using a thresholding approach, and compared SN volume and T1 values between healthy controls and patients with Parkinson's disease.