The purpose of this study is to establish the reliability of the UTE-T2* mapping method using 3DCones sequence shown to detect subsurface changes in knee cartilage and meniscus. Scans were performed on cadaver knee specimen using 3DCones acquisition at 3T with 8 and 12 TEs. Reliability was assessed by the intraclass correlation coefficient (ICC) calculated as 0.77 and 0.91 for the 8 and 12 point techniques. The method is good to excellently reproducible for both techniques. Hence, the 3DCones UTE-T2* mapping technique can reliably evaluate subsurface changes in meniscus and cartilage and help identify at-risk OA patients.