The most common method for fat-water separation nowadays is chemical shift-encoded imaging (CSEI). However, when studying fat accumulation in skeletal muscles and high spatial resolution fat fraction (FF) images are desired, CSEI might be challenging due to the increased inter-echo spacing. Here, three alternative methods based on T2-relaxation times, have been explored and compared to CSEI by calculating the muscular fat fraction of ten healthy volunteers. All T2-based methods resulted in qualitatively improved high-resolution FF images compared to CSEI, while a 2-parameter non-linear least square fit showed best quantitative agreement to low resolution CSEI.
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