A setup for operant learning fMRI was developed and inserted in a horizontal bore 14 T MRI. After the habituation of head-fixed mice, visual stimulation was delivered as CS and a water as reward was supplied automatically in response to licking behavior, for an operant learning task. We analyzed fMRI data between the correct and the error trials and found the BOLD elevation of brain areas including the visual cortex and the hippocampal formation when mice performed the correct trial. Mouse BOLD fMRI in operant learning task will offer unexperienced data to basic as well as clinical research fields.
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