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Abstract #5619

The effect of malonate, succinate, oxaloacetate and 2-deoxy-D-glucose on boar sperm metabolism using 13C MRS

Nurul Fadhlina Ismail1, Steven Reynolds1, Sarah Calvert2, Allan Pacey2, and Martyn Paley1

1Academic Unit of Radiology, University of Sheffield, Sheffield, United Kingdom, 2Academic Unit of Reproductive & Developmental Medicine, University of Sheffield, Sheffield, United Kingdom

One in five young men has poor semen quality, including low motility. Studying energy metabolism may provide better understanding of sperm motility. We acquired 13C Magnetic Resonance spectra of sperm incubated with 13C-glucose with different concentrations of inhibitors: malonate, oxaloacetate, succinate, and 2-deoxy-D-glucose. This study examined the effect of these inhibitors on sperm lactate production and vitality, with a secondary aim to observe Krebs cycle intermediates in the MR spectrum. Glucose signal significantly decreased with increasing oxaloacetate concentration. Malonate and oxaloacetate and 2DG significantly decreased in lactate production. These inhibitors did not lead to observable 13C labelled Krebs cycle intermediates.

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