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Abstract #0154

Mesoscopic and microscopic imaging of sensory responses in the same animal

Davide Boido1, Ravi L Rungta1, Bruno-Felix Osmanski2, Morgane Roche1, Tomokazu Tsurugizawa3, Denis LeBihan3, Luisa Ciobanu3, and Serge Charpak1

1INSERM U1128, Laboratory of Neurophysiology and New Microscopy, Université Paris Descartes, Paris, France, 2INSERM U979 ‘Wave Physics for Medicine’ Lab, Paris, France, 3NeuroSpin, Bât 145, Commissariat à l'Energie Atomique-Saclay Center, Gif-sur-Yvette, France

We developed a chronic olfactory bulb preparation compatible with repetitive imaging of the same mice with BOLD-fMRI (17.2 T), functional ultrasound imaging (fUS) and two-photon laser scanning microscopy. BOLD-fMRI and fUS mesoscopic signals are highly correlated with microscopic vascular and dendritic neuronal signals in response to odour concentrations. Furthermore, minimal odour stimulation reveals that there is no threshold of neuronal activation below which functional hyperemia is not triggered, warranting measurement of blood flow dynamics to detect the lowest levels of brain activation. These data establish the strengths and limits of mesoscopic imaging techniques to report neural activity.

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