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Abstract #0399

Active Targeting of human neutrophil granulocytes by non-invasive 19F MRI

Pascal Bouvain1, Bodo Steckel1, Wolfgang Krämer2, Rolf Schubert2, Sebastian Temme1, and Ulrich Flögel1

1Heinrich-Heine University, Düsseldorf, Germany, 2Albert-Ludwigs-University, Freiburg, Germany

The purpose of the present study was to target human neutrophils by PFCs to enable their visualization by 19F MRI. We coupled a neutrophil-binding peptide (NG2) to PFCs and showed the specific binding and internalization of NG2-PFCs by 19F MRI, microscopy and FACS. Interestingly, NG2-PFCs show an increased labelling of neutrophils from MI patients and these cells also showed an increased migration into an artificial circulation system which contained an IL-8 doped matrigel placed in a flow chamber. In conclusion, NG2-PFCs are suited to label neutrophil granulocytes after MI and enable their non-invasive visualization by 1H/19F MRI.

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