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Abstract #1007

Simultaneous fMRI and multispectral fiber-photometry reveals neurovascular coupling during somatosensory, optogenetic, and chemogenetic stimulation of S1 principle neurons

Tzu-Hao Harry Chao1, Weiting Zhang1, Brittany Katz1, Esteban Oyarzabal1, Sung-Ho Lee1, Guohong Cui2, and Yen-Yu Ian Shih1

1Biomedical Research Imaging Center and Department of Neurology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States, 2Neurobiology Laboratory, National Institute of Environmental Health Sciences, NIH, Durham, NC, United States

In this study, we establish an experimental platform to simultaneously measure: a) genetically encoded calcium indicators (GCaMP) expressing on the excitatory neurons using fiber-photometry, b) cerebral blood volume (CBV) using fiber-photometry, and c) CBV using fMRI. By this platform, we assess neurovascular coupling (GCaMP and CBV comparisons) under chemogenetic stimulation of S1 excitatory neurons in a group of freely moving rats, and demonstrate this platform for concurrent GCaMP, photometry-CBV, and fMRI-CBV measurements with chemogenetic manipulation.

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