Abstract #1007

Simultaneous fMRI and multispectral fiber-photometry reveals neurovascular coupling during somatosensory, optogenetic, and chemogenetic stimulation of S1 principle neurons

Tzu-Hao Harry Chao¹, Weiting Zhang¹, Brittany Katz¹, Esteban Oyarzabal¹, Sung-Ho Lee¹, Guohong Cui², and Yen-Yu Ian Shih¹

¹Biomedical Research Imaging Center and Department of Neurology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States, ²Neurobiology Laboratory, National Institute of Environmental Health Sciences, NIH, Durham, NC, United States

In this study, we establish an experimental platform to simultaneously measure: a) genetically encoded calcium indicators (GCaMP) expressing on the excitatory neurons using fiber-photometry, b) cerebral blood volume (CBV) using fiber-photometry, and c) CBV using fMRI. By this platform, we assess neurovascular coupling (GCaMP and CBV comparisons) under chemogenetic stimulation of S1 excitatory neurons in a group of freely moving rats, and demonstrate this platform for concurrent GCaMP, photometry-CBV, and fMRI-CBV measurements with chemogenetic manipulation.

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