This study measures alterations in wholebrain connectivity and connectivity of the suspected seizure onset zone (SOZ) in MRI-negative epilepsy patients. Short-range (U-fibers), long-range, and inter-limbic white matter tracts were measured independently in a group of 19 epilepsy subjects and 10 healthy controls using diffusion imaging. Relative wholebrain hypo-connectivity of both U-fibers and inter-limbic fibers was found in epilepsy subjects. Additionally, hyper-connectivity of all 3 fiber types was found in SOZ regions compared with non-SOZ regions. These findings suggest abnormal connectivity in MRI-negative patients may prove useful in SOZ localization and treatment planning.