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Abstract #1417

Elevated conversion of hyperpolarized [1-13C]pyruvate to [1-13C]lactate is not associated with tissue acidosis, as measured with hyperpolarized [13C]bicarbonate, in a murine model of rheumatoid arthritis.

Alan J. Wright1, Zoé M. A. Husson2, De-en Hu1, Gerard Callejo2, Kevin M. Brindle1,3, and Ewan St. John Smith2

1CRUK Cambridge Institute, University of Cambridge, Cambridge, United Kingdom, 2Department of Pharmacology, University of Cambridge, Cambridge, United Kingdom, 3Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom

Measurements of synovial fluid pH in patients with rheumatoid arthritis suggest acidosis can occur at inflamed joints. A widely used model of rheumatoid arthritis is produced by injecting complete Freund’s adjuvant into the hind paw of a mouse. We have investigated whether inflammation is associated with acidosis in this model using Magnetic Resonance Spectroscopic Imaging of injected hyperpolarised [1-13C]pyruvate, to detect the metabolic changes associated with inflammation, and hyperpolarised [13C]bicarbonate to measure extracellular pH. A significant increase in the [1-13C]lactate/[1-13C]pyruvate was observed throughout the inflamed tissue, but there is no apparent acidosis

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