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Abstract #2066

Effect of autolysis, fixation, and storage in PBS on relaxation rates and macro-molecular tissue volume across fiber pathways of the human brain

Mohammad Ashtarayeh1, Tobias Streubel1, Klaus Püschel2, and Siawoosh Mohammadi1

1Department of Systems Neuroscience, Medical Center Hamburg-Eppendorf, Hamburg, Germany, 2Center for Diagnostics, Institute of Legal Medicine, Medical Center Hamburg-Eppendorf, Hamburg, Germany

We evaluated the effect of autolysis, brain tissue fixation, and embedding into PBS on three potential quantitative myelin MRI markers across different white matter fiber pathways: longitudinal (R1) and effective transverse (R2*) relaxation rates, and macro-molecular tissue volume (MTV) using the quantitative multi-parameter mapping (MPM) protocol. We found that the effect of autolysis was most apparent in R2* and MTV, R1 drastically changed its contrast after fixation, and R1 and R2* values increased after storage in Phosphate-Buffered Saline (PBS) solution.

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