Abstract #2319

Investigating the Functional Diffusion-Signal Response (DfMRI) in Living, CA1 Pyramidal Neurons Undergoing Chemical Activation with Kainate

Jeremy J. Flint^1,2, Kannan Menon^2,3,4, Brian Hansen⁵, John Forder^2,3,4, and Stephen J. Blackband^1,2,6

¹Neuroscience, University of Florida, Gainesville, FL, United States, ²McKnight Brain Institute, University of Florida, Gainesville, FL, United States, ³Biomedical Engineering, University of Florida, Gainesville, FL, United States, ⁴Radiology, University of Florida, Gainesville, FL, United States, ⁵Center for Functionally Integrative Neuroscience, Aarhus University, Aarhus, Denmark, ⁶National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL, United States

In the current study, we use MR microscopy and superfusion techniques to investigate changes in the diffusion properties of living, hippocampal neurons following activation with kainate. Acute hippocampal slice preparations (n = 6) were imaged at six different b values (b = 0 to 3200 s/mm²) both before and after exposure to kainate (100mM). Significant activation-based changes (p = 0.0043) in diffusion properties were detected in the stratum pyramidale: the tissue lamina comprised primarily of pyramidal neuron cell bodies. No changes were observed in either the strata oriens (apical dendrites) or radiatum (axons).

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