In-vivo radiological imaging is used globally to detect possible cancers and inform treatment decisions, but difficulties arise when attempting to compare radiological findings to the gold-standard of diagnosis, histopathology. Standard imaging protocols have documented success but to determine the reliability of new imaging sequences and modalities, correlation to histopathology must be made. Several methods have been proposed for registration in both 2D and 3D, but these have shown limited effectiveness and often require unique equipment or proprietary algorithms. In this study, we attempt to complete an accurate registration in 2D in order to validate different imaging modalities.
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