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Abstract #3566

Probing renal pH and aminopeptidase N activity with hyperpolarized [1-13C]alaninamide

Alice Radaelli1, Hikari Ananda Infinity Yoshihara1, Ryunosuke Hata2, Shinsuke Sando3, and Rolf Gruetter1,4

1Laboratory for Functional and Metabolic Imaging (LIFMET), EPFL, Lausanne, Switzerland, 2Department of Chemistry and Biochemistry, Kyushu University, Fukuoka, Japan, 3Department of Chemistry and Biotechnology, The University of Tokyo, Tokyo, Japan, 4Center for Biomedical Imaging (CIBM), Lausanne, Switzerland

The detection of aminopeptidase N (APN) activity can give information on tumor development. Hyperpolarized L-[1-13C]alaninamide is a specific, sensitive probe of APN activity in kidney homogenate. Here, we characterized its in vivo metabolic response in the rat kidney. In addition to being an APN substrate, L-[1-13C]alaninamide is sensitive to pH and also reacts with dissolved carbon dioxide. To avoid spectral overlap, alaninamide is best suited as an APN probe in acidic environments, and it may have additional applications as a multifunctional sensor of pH and CO2.

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