Abstract #3566

Probing renal pH and aminopeptidase N activity with hyperpolarized [1-13C]alaninamide

Alice Radaelli¹, Hikari Ananda Infinity Yoshihara¹, Ryunosuke Hata², Shinsuke Sando³, and Rolf Gruetter^1,4

¹Laboratory for Functional and Metabolic Imaging (LIFMET), EPFL, Lausanne, Switzerland, ²Department of Chemistry and Biochemistry, Kyushu University, Fukuoka, Japan, ³Department of Chemistry and Biotechnology, The University of Tokyo, Tokyo, Japan, ⁴Center for Biomedical Imaging (CIBM), Lausanne, Switzerland

The detection of aminopeptidase N (APN) activity can give information on tumor development. Hyperpolarized L-[1-¹³C]alaninamide is a specific, sensitive probe of APN activity in kidney homogenate. Here, we characterized its in vivo metabolic response in the rat kidney. In addition to being an APN substrate, L-[1-¹³C]alaninamide is sensitive to pH and also reacts with dissolved carbon dioxide. To avoid spectral overlap, alaninamide is best suited as an APN probe in acidic environments, and it may have additional applications as a multifunctional sensor of pH and CO₂.

How to access this content:

For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.

After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.

After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.

Click here for more information on becoming a member.