While BLI is very complementary to MRI in evaluating the fate of many different cell populations in vivo, including cancer cells, some considerations for the use of BLI have been reported including an increase in tumor growth variation as well as a change in metastatic pattern following luciferase tagging. The objective of this work was to use cellular and anatomical MRI to characterize the in vivo growth patterns of naive and lentiviral-engineered brain-seeking breast cancer cell lines co-expressing fluorescent and bioluminescent reporters in the mouse brain.
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