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Abstract #3885

Measurement of oxygen consumption in a high density 3D cell culture on chip by 19F spectroscopy

Dennis Kleimaier1, Michaela Hoesl1, Andreas Neubauer1, Matthias Malzacher1, Cordula Nies2, Eric Gottwald2, and Lothar Schad1

1Computer Assisted Clinical Medicine, Heidelberg University, Mannheim, Germany, 2Institute of Functional Interfaces, Karlsruhe Institute of Technology, Karlsruhe, Germany

Drug-induced mitochondrial dysfunction is of major clinical interest. An NMR-compatible bioreactor system was used to investigate the oxygen consumption of HepG2 cells by measuring T1 of perflubron. The oxygen consumption was measured by stopping the perfusion. This resulted in a reduction of the oxygen concentration from (19.29 ± 0.96)% to (11.41 ± 0.92)% in 104.24 min. Our results might allow for detecting a drug-induced mitochondrial dysfunction of cells in a well-controlled environment by using the two compartments of the bioreactor.

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