Abstract #3885

Measurement of oxygen consumption in a high density 3D cell culture on chip by 19F spectroscopy

Dennis Kleimaier¹, Michaela Hoesl¹, Andreas Neubauer¹, Matthias Malzacher¹, Cordula Nies², Eric Gottwald², and Lothar Schad¹

¹Computer Assisted Clinical Medicine, Heidelberg University, Mannheim, Germany, ²Institute of Functional Interfaces, Karlsruhe Institute of Technology, Karlsruhe, Germany

Drug-induced mitochondrial dysfunction is of major clinical interest. An NMR-compatible bioreactor system was used to investigate the oxygen consumption of HepG2 cells by measuring T₁ of perflubron. The oxygen consumption was measured by stopping the perfusion. This resulted in a reduction of the oxygen concentration from (19.29 ± 0.96)% to (11.41 ± 0.92)% in 104.24 min. Our results might allow for detecting a drug-induced mitochondrial dysfunction of cells in a well-controlled environment by using the two compartments of the bioreactor.

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