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Abstract #3895

Investigation of ethanol stimulation on HepG2 cells in a high density cell culture on chip by correlation spectroscopy

Dennis Kleimaier1, Andreas Neubauer1, Michaela Hoesl1, Cordula Nies2, Eric Gottwald2, and Lothar Schad1

1Computer Assisted Clinical Medicine, Heidelberg University, Mannheim, Germany, 2Institute of Functional Interfaces, Karlsruhe Institute of Technology, Karlsruhe, Germany

Ethanol-induced toxicity leads to alterations of certain cellular functions such as mitochondrial dysfunction. This study investigated the feasibility to detect changes induced by 2‰ ethanol stimulation on HepG2 cells in an NMR-compatible bioreactor system by double quantum filtered correlation spectroscopy. During ethanol stimulation, the spectra with cells led to an increased SNR of the glucose (15.5±2.1 %) and lactic acid (10.8%) cross peaks. These changes were larger than the SNR reproducibility of (3.94±2.44)%. Our results show that the DQF-COSY sequence can be used for the investigation of ethanol-induced damage on cells in a bioreactor system (worse shim condition).

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