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Abstract #4013

NMR-BASED METABOLOMIC ANALYSIS AS A DIAGNOSTIC TOOL FOR TUBERCULOSIS IN CLINICAL URINE SAMPLES

Jose Luis Izquierdo-Garcia1,2, Ramon Campos-Olivas3, Mar Serra4,5, Cristina Prat4,5, Jose Dominguez4,5, and Jesus Ruiz-Cabello2,6

1CNIC, Madrid, Spain, 2CIBERES, Madrid, Spain, 3CNIO, Madrid, Spain, 4Servicio de Microbiologia. Hospital Universitari Germans Trias i Pujol. Institut d'Investigació Germans Trias i Pujol. Universitat Autònoma de Barcelona, Barcelona, Spain, 5CIBERES, Barcelona, Spain, 6Universidad Complutense de Madrid, Madrid, Spain

The ability of diagnose the tuberculosis infection is an essential factor in the spreading control of tuberculosis. However, microscopic examination presents a low sensitivity and culture techinques require incubation times up to two months. This study aimed at developing a NMR-based metabolomic approach for the differential diagnosis of tuberculosis in urine samples. We examined samples from patients diagnosed of tuberculosis (n=19), other respiratory infection (n=25) and healthy controls (n=29). Unsupervised PCA provide a nearly perfect discrimination between the three groups. We identified 31 chemical shifts regions to develop predictive models for the diagnostic of tuberculosis, obteining an accuracy of 100%.

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