Clinical knee MRI is most commonly performed with 2D-turbo-spin-echo (TSE) sequences in multiple imaging planes. Although these multi-slice sequences provide excellent tissue contrast and high in-plane spatial resolution, they use relatively thick slices, which leads to partial volume effects. This study used super-resolution reconstruction (SRR) to reconstruct isotropic high-resolution knee MR images. Our results indicate that SRR of knee MRI is feasible and compares favorably to direct high-resolution TSE acquisition in terms of image quality and acquisition time. SRR provides an attractive alternative for isotropic 3D-MRI of the knee.