Life-long premature ejaculation (LPE) is a common male sexual dysfunction. Previous studies revealed ejaculation-related control mechanisms in the brain. However, whether this control network changes in patients with LPE is unclear. A stop signal test was used in this study. A study of task-related functional MRI found there was a significant difference in neural network activation between healthy controls and LPE patients during go process. This study indicates a neural network abnormality in LPE patients, which provides novel reference for understanding the neural mechanism of LPE.