The impact of temperature on inhomogeneous magnetization transfer (ihMT) measurements in formalin-fixed human brain was studied. White matter (WM) ihMT signal increased with temperature when a T1D-filter was applied, supporting the hypothesis that components with long T1D (>1ms) are more sensitive to temperature variations. Grey matter ihMT did not vary with temperature. This suggests a difference in T1D values between WM and GM, and confirms that several T1D components contribute to myelin ihMT. Myelin water fraction values decreased with increasing temperature, possibly due to faster exchange between water compartments. Temperature is an important factor to consider in order to characterize the microstructure.