Contrast enhanced MRI studies are currently limited to observing a single contrast agent at a time. Meanwhile, optical imaging methods frequently utilize multiple fluorophores in “multi-color” imaging studies to track multiple molecular events simultaneously. We propose the novel DC-MRF method as a way to realize an analagous “multi-color” MRI. Herein, we demonstrate the first in vivo application of multi-color MRF and validate its ability to accurately and dynamically map the concentration of multiple contrast agents in vivo. This method creates the opportunity to perform a wide range of novel multi-agent MRI studies that immediately broadens the scope of contrast enhanced MRI.
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