Histological necrosis is the current gold standard for response evaluation in osteosarcoma treated with neoadjuvant chemotherapy (NACT). However it is applicable only after tumor resection on completion of NACT. Thus, a non-invasive early marker of NACT response is desirable. We performed NACT response prediction and evaluation using Intra-voxel Incoherent Motion (IVIM) Diffusion weighted MRI and histogram analysis. IVIM parameters and its histogram analysis revealed clinically useful information in characterizing and predicting chemotherapy response to Osteosarcoma.