We applied novel RAFFn relaxation times (TRAFF2 and TRAFF4), T1ρ and T2 to study effects of cardiac lymphatic system in myocardial infarct (MI). Infarct size based on the TRAFF4 relaxation time maps was significantly larger in earlier time points post MI in wild-type compared to lymphatic insufficient mice groups. No differences were found in relaxation times between groups. Area-of-overestimation (AOE) values remained stable in lymphatic insufficient group while in wild-type group a decreasing trend of AOE was observed. We conclude that effects of lymphatics after MI can be detected based on infarct size difference measured with different relaxation times.