A different level of overlap between intramyocellular and extramyocellular lipids signal (EMCL and IMCL) depends on muscle fiber orientation and interstitial/fascial muscle fat tissue. We proposed diffusion tensor imaging and Dixon reconstructed fiber orientation map and maximum intensity projection fat maps to guide MR spectroscopy acquisition by carefully positioning the voxel so as to minimize these two effects. This method can be used to improve the consistency and accuracy of using MRS to quantify the intra skeletal muscle lipids and minimize variability in longitudinal studies such as evaluating disease progression or monitoring treatment progress
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