In vivo MRI was used to detect and measure the brain hemodynamic action of carbamoylated erythropoietin (Cepo) in mice. Brain perfusion was measured using dynamic susceptibility contrast MRI, and BBB permeability was detected by pre- and post-contrast T1 mapping. It was found that Cepo caused increased cerebral blood flow and volume. Consistent pre- and post-contrast T1 values indicated no gadolinium leakage from vascular system to brain tissue. In summary, chronic Cepo treatment induced increased brain perfusion and this can be detected using in vivo MRI.