The impairment of microvessels can lead to neurologic diseases such as stroke and vascular dementia. The imaging of lumen and vessel wall of perforating arteries requires an extremely high resolution due to their small caliber size (50 – 400 um). In this study, we developed a 3D inner-volume (IV) TSE (SPACE) sequence with 2D spatially selective excitation (SSE) RF pulses. High resolution of isotropic 0.30mm was achieved for the black-blood images of lenticulostriate artery (LSA) within 10 minutes. The IV-SPACE images showed clearer delineation of vessel wall and lumen of LSA than conventional SPACE images. IV-SPACE might be a promising method for detecting microvasculopathies of cerebral vascular diseases.