Double diffusion encoding (DDE) allows the estimation of microscopic fractional anisotropy (μFA), which is a promising metric for studying microstructural properties of brain tissue independent of orientation dispersion. However, a large number of acquisitions is needed to obtain a rotationally invariant measurement of μFA, which poses a problem in clinical settings. A DDE protocol with a reduced number of acquisitions has recently been proposed as a solution. In this study, we assessed the accuracy of this approach and its potential for reporting μFA accurately. Our results show that a reduced number of acquisitions is sufficient for characterizing μFA.
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