Microbubble-mediated focused ultrasound can noninvasively induce reversible blood-brain barrier (BBB) opening in both rodents and non-human primates. However, it remains unclear whether FUS-induced BBB opening is accompanied by neuromodulation. Here we longitudinally characterized the duration and the functional effects of FUS-induced BBB opening by measuring changes in contrast-enhanced T1-weighted images (T1-WIs) and blood-oxygen-level dependent (BOLD) responses, respectively. The results show that BBB recovered at 6.5h post-FUS exposure. The blood-oxygen-level dependent (BOLD) changes during the visual stimulus pre- and post- FUS exposure didn't show similar change trend in both hemispheres. The results of fMRI-guided FUS may contribute to the development of FUS-induced BBB opening for clinical applications.