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Abstract #3961

The contribution of cells and extracellular matrix to liver viscoelasticity: Histological analysis combined with multifrequency MR elastography and diffusion weighted imaging in rat liver specimens

Ingolf Sack1, Heiko Tzschätzsch1, Baptiste Polchlopek1, Paul Janmey2, Jürgen Braun1, and Angela Ariza de Schellenberger1

1Radiology, Charité-Universitätsmedizin Berlin, Berlin, Germany, 2University of Pennsylvania, Philadelphia, PA, United States

The effect of freezing/thawing at -20°C and -80°C on liver tissue was analyzed by diffusion-weighted imaging (DWI), multifrequency MR elastography (MRE) and histological methods. Freezing-induced deterioration of cell membranes, detachment of sinusoidal endothelial cells and disorganization of sinusoidal collagen together with reduced water diffusion and macroscopic shear modulus and affected tissue viscosity. Histological analysis revealed that collagen structures at the space of Disse are better preserved at -80°C than -20°C which was mirrored by tissue viscosity indicating the sensitivity of multifrequency MRE to microstructural changes of soft biological tissues.

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