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Abstract #4000

Initial CEST study in a microcavity array based 3D cell culture system

Dennis Kleimaier1, Steffen Goerke2, Cordula Nies3, Eric Gottwald3, and Lothar Schad1

1Computer Assisted Clinical Medicine, Heidelberg University, Mannheim, Germany, 2Division of Medical Physics in Radiology, German Cancer Research Center (DKFZ), Heidelberg, Germany, 3Institute of Functional Interfaces, Karlsruhe Institute of Technology, Karlsruhe, Germany

Chemical exchange saturation transfer (CEST) is a powerful method for the investigation of biomolecules. An MR-compatible bioreactor, on the other hand, creates a well-controlled environment for human cells. The combination of both methods should make it possible to extract cellular signals from the bioreactor. Therefore, we investigated the possibility of performing CEST measurements in an actively-perfused bioreactor with HepG2 cells. We could show that perfusion had no effect on the CEST signals and that the main signals originated from the cells. However, the sub-optimal shim condition in the bioreactor led to a reduction in CEST signals.

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