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Abstract #4218

Ubiquitous Mitochondrial Creatine Kinase (CKMT1) Drives Malignant Creatine Metabolite Profiles in Human Breast Cancer Cell Lines

Vinay Ayyappan1, Caitlin Tressler1, Menglin Cheng1, Kanchan Sonkar1, Ruoqing Cai1, Michael T. McMahon2, and Kristine Glunde1

1Johns Hopkins University School of Medicine, Baltimore, MD, United States, 2F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD, United States

Ubiquitous Mitochondrial Creatine Kinase (CKMT1) is a mitochondrial membrane protein that catalyzes the reversible conversion of creatine (Cr) to phosphocreatine (PCr). This study shows that in two triple-negative breast cancer cell lines, CKMT1-overexpression resulted in significant increases in intracellular Cr and PCr and conferred increases in cell viability while decreasing migration, invasion and adhesion. Since CKMT1 has been identified as a prognostic indicator in several cancers, it may play a role in oncogenesis and/or cancer progression. Thus, CKMT1 holds promise as a potential diagnostic marker and/or treatment target whose expression can be monitored using magnetic resonance spectroscopy-detected Cr and PCr levels.

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