This study employed high-resolution 3D-MRI to map the strain of the eyeball during adduction and abduction tasks. The strain map is highly heterogeneous with high strain toward the anterior region. Adduction induced higher strain than abduction, as expected due to more stretching of the optic nerve in the adduction position. This is the first MRI measurement of strain of the eyeball. This approach could have clinical applications in eye movement disorders and eye diseases.