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Abstract #0125

The immune checkpoint PD-L1 alters choline kinase expression and metabolism in triple negative breast cancer cells

Jesus Pacheco-Torres1, Marie-France Penet1,2, Flonne Wildes1, Yelena Mironchik1, Balaji Krishnamachary1, and Zaver M Bhujwalla1,2,3
1The Russell H Morgan Department of Radiology and Radiological Science, The Johns Hopkins University, School of Medicine, Baltimore, MD, United States, 2Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University, School of Medicine, Baltimore, MD, United States, 3Radiation Oncology and Molecular Radiation Sciences, The Johns Hopkins University, School of Medicine, Baltimore, MD, United States

Expression of programmed death-ligand 1 (PD-L1) plays a significant role in creating an immune suppressive tumor microenvironment. We investigated the relationship between the aberrant choline metabolism observed in most cancers and PD-L1 expression in triple negative human MDA-MB-231 breast cancer cells. Using siRNA to downregulate Chk-a or PD-L1 or both, we identified a close inverse interdependence between Chk-α and PD-L1. We identified, for the first time, the role of PD-L1 in cancer cell metabolism. These results have significant implications for therapy and provide new insights into the relationship between metabolism and immune resistance in these breast cancer cells.

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